The nucleotide sequence and derived amino acid sequence of eDNA coding for mouse carbonic anhydrase II (Comparison with mammalian carbonic anhydrase isozymes; evolution; unique residues; long 3' noneoding

The nucleotide sequence of a clone containing mouse carbonic anhydrase (CA) eDNA in pBR322 has been determined. The cloned eDNA contains all of the coding region except for nueleotides specifying the first eight amino acids, and all of the 3' noncoding region, which consists of 700 nucleotides. A eDNA clone was identified which contains an additional 54 bp at the 5' end, so that the complete amino acid sequence of mouse CA could be deduced. This sequence showed a 73-81% homology with other mammalian CA form II isozymes, 56--63 with form I isozymes, and 52-56 ~o with form III isozymes. By examination of the amino acids which are unique and invariant for each isozyme, the mouse amino acid sequence was found to contain 16 of the 23 residues that are unique and invariant to mammalian CA form II isozymes, but only one or no residue for forms I and III, respectively.